In vitro effects of a commercial blend of functional oils on fermentation, methane production and responsible methanogenic archaea
| Autor: | Seradj, Ahmad Reza, Torrent, J., de la Fuente Oliver, Gabriel, Balcells Terés, Joaquim |
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| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Repositorio Abierto de la UdL Universitad de Lleida Recercat. Dipósit de la Recerca de Catalunya instname Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Popis: | A complete randomized block design trial with 4 in vitro incubation sets were prepared to evaluate the effect of a commercial blend of functional oils (FO; Oligo Basics, Brazil) on rumen fermentation, methane production and methanogenic archaea. Bottles were filled with concentrate diets and an incubation solution under a C02 stream. Sealed bottles were incubated for 24 h and either dosed with 500 µg of FO/gDM of basal diet or not (control). The headspace pressure was measured and sampled at 2 h intervals, up to 12 h and 24 h post incubation to determine gas and then methane concentration using GC. After DNA extraction, specific primers were used to determine absolute abundance of methanogenic archaea (MA) and the relative abundance of MA in relation to total archaea using qPCR. Bottles supplemented with FO showed a tendency (14.26 vs. 13.50) to decrease methane production and reduced the methane ratio (CH4/gas; 0.093 vs. 0.089; P=0.05), where the discrete lag time for gas production increased (0.04 vs.0.12; P=0.047). Addition of FO improved rumen fermentation, increasing molar proportion of propionate (33.5 vs. 34.3; P=0.024) and decreasing NH3-N concentration (305.3 vs. 284.4; P=0.022) and relative abundance of MA (17.8 vs. 14.7; P=0.03). |
| Databáze: | OpenAIRE |
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