Fate of insecticidal Bacillus thuringiensis cry protein in soil: Differences between purified toxin and biopesticide formulation

Autor: Truong Phuc, Hung, Le Van, Truong, Binh, Ngo Dinh, Frutos, Roger, Quiquampoix, Herve, Staunton, Siobhan
Přispěvatelé: Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institute of Biotechnology, Vietnam Academy of Sciences and Technology, Université de Montpellier (UM), Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université de Bordeaux (UB), Programme GESSOL du projet EBISOL project (MEDDE), bourse Erasmus Mundus du projet MAHEVA European Commission (EACEA)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Pest Management Science
Pest Management Science, Wiley, 2016, 72 (12), pp.2247-2253. ⟨10.1002/ps.4262⟩
Pest Management Science 12 (72), 2247-2253. (2016)
Pest Management Science, 2016, 72 (12), pp.2247-2253. ⟨10.1002/ps.4262⟩
ISSN: 1526-498X
1526-4998
DOI: 10.1002/ps.4262⟩
Popis: International audience; Background :Bacillus thuringiensis produces insecticidal proteins known as Cry and its efficiency and absence of side-effects make it the most widely used biopesticide. There is little information on the role of soils in the fate of Cry proteins from commercial biopesticide formulations, unlike toxins from genetically modified crops that have been intensively studied in recent years. The persistence of Cry in soil was followed under field and laboratory conditions. Results : Sunlight accelerated loss of detectable Cry under laboratory conditions but little effect of shade was observed under field conditions. The half-life of biopesticide proteins in soil under natural conditions was about one week. Strong temperature effects were observed, but they differed for biopesticide and purified protein, indicating different limiting steps. Conclusion : For the biopesticide the observed decline in detectable protein was due to biological factors, possibly including the germination of B. thuringiensis spores and was favoured by higher temperature. In contrast for purified proteins, the decline in detectable protein was slower at low temperature, probably because the conformational changes of the soil-adsorbed protein, that cause fixation and hence reduced extraction efficiency, are temperature dependent.
Databáze: OpenAIRE
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