Transcriptomic analysis of the highly efficient oil-degrading bacterium Acinetobacter venetianus RAG-1 reveals genes important in dodecane uptake and utilization
| Autor: | Kothari, A, Charrier, M, Wu, YW, Malfatti, S, Zhou, CE, Singer, SW, Dugan, L, Mukhopadhyay, A |
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| Přispěvatelé: | Heipieper, Hermann |
| Rok vydání: | 2016 |
| Předmět: |
Acinetobacter
Acinetobacter venetianus RAG-1 ATCC 31012 Agricultural and Veterinary Sciences Gene Expression Profiling Bacterial Membrane Transport Proteins Biological Transport DNA transcriptomic Acetates Biological Sciences alkane uptake alkane monooxygenase Microbiology Medical and Health Sciences Environmental Fimbriae Dodecanol Alkanes dodecane Biodegradation alkane hydroxylase Genetics Petroleum Pollution Sequence Analysis |
| Zdroj: | FEMS microbiology letters, vol 363, iss 20 Kothari, A; Charrier, M; Wu, YW; Malfatti, S; Zhou, CE; Singer, SW; et al.(2016). Transcriptomic analysis of the highly efficient oil-degrading bacterium Acinetobacter venetianus RAG-1 reveals genes important in dodecane uptake and utilization. FEMS Microbiology Letters, 363(20). doi: 10.1093/femsle/fnw224. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/1nh7p66d |
| DOI: | 10.1093/femsle/fnw224. |
| Popis: | © FEMS 2016. All rights reserved. The hydrocarbonoclastic bacterium Acinetobacter venetianus RAG-1 has attracted substantial attention due to its powerful oil-degrading capabilities and its potential to play an important ecological role in the cleanup of alkanes. In this study, we compare the transcriptome of the strain RAG-1 grown in dodecane, the corresponding alkanol (dodecanol), and sodium acetate for the characterization of genes involved in dodecane uptake and utilization. Comparison of the transcriptional responses of RAG-1 grown on dodecane led to the identification of 1074 genes that were differentially expressed relative to sodium acetate. Of these, 622 genes were upregulated when grown in dodecane. The highly upregulated genes were involved in alkane catabolism, along with stress response. Our data suggest AlkMb to be primarily involved in dodecane oxidation. Transcriptional response of RAG-1 grown on dodecane relative to dodecanol also led to the identification of permease, outer membrane protein and thin fimbriae coding genes potentially involved in dodecane uptake. This study provides the first model for key genes involved in alkane uptake and metabolism in A. venetianus RAG-1. |
| Databáze: | OpenAIRE |
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