| Popis: |
Explant Culture of Trifolium pratense L. A principial precondition for successful elicitation used to increase the production of secondary metabolities is, among other, finding a suitable elicitor, its concentration and the optimal period of time of the action of the elicitor on the plant culture in vitro, which was the aim of the present thesis. The effect was examined of a 6, 24, 48 and 168 hour action of the solution of mercury dichloride (in four concentrations) on the production of flavonoids and isoflavonoids in the callus and suspension culture of Trifolium pratense L. cultivated on a Gamborg medium with an addition of 2 mg.l-1 of 2,4-dichlorophenoxyacetic acid and 2 mg.l-1 of 6-benzylaminopurine, at the temperature of 25řC and 16 hour light/8 hour dark period. The maximal content of flavonoids found by a photometric determination according to Pharmacopoeia Bohemica 2005 was demonstrated in the callus culture (0,636 %) after 6 hour action of the elicitor of the 100 µmol concentration and in the suspension culture (0,492 %) after 168 hour action of the elicitor of the 10 µmol concentration. The maximal content of isoflavonoids found by a HPLC method was demonstrated in the suspension culture after 168 hour action of the elicitor of the 100 µmol concentration (0,03 % of daidzein) and after 168... |
