Fast evolution of Interleukine-2 in mammals, ans positive selection in ruminants

Autor: Zelus, D., Delacre, M., Robinson, M., Auriault, C., Laudet, Vincent
Přispěvatelé: Laboratoire de Biologie Moléculaire de la Cellule (LBMC), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Bigouraux, Sylvie
Jazyk: angličtina
Rok vydání: 2000
Předmět:
Zdroj: Journal of Molecular Evolution
Journal of Molecular Evolution, 2000, 51, pp.234-244
ISSN: 0022-2844
1432-1432
Popis: International audience; Interleukin-2 (IL-2) is a cytokine involved in induction and regulation of the immune response in mammals. There have been numerous reports about the search for IL-2 in species other than mammals, and recently an IL-2-like gene has been isolated in chicken. Using PCR, we searched for IL-2 gene sequences in a wide variety of mammals, including marsupials and monotremes, as well as in birds. Although we can readily amplify IL-2 gene fragments in placental mammals, no amplification was obtained in other species. This is best explained by very high substitution rates. This suggest that strategies to isolate IL-2 homologous genes outside mammals should involve functional assays, as for the chicken gene, and not hybridization-based techniques. Nonsynonymous substitution rates are especially high in ruminants, due to positive selection acting on regions important in term of structure-function. We suggest that, although globally similar, the immune response of various mammals is not identical, mainly at the level of cytokine-mediated regulations.
Databáze: OpenAIRE
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