In Vitro Bone Cell Behavior on Porous Titanium Samples: Influence of Porosity by Loose Sintering and Space Holder Techniques
| Autor: | Civantos, Ana, Giner García, Mercedes, Trueba Muñoz, Paloma, Lascano, Sheila, Montoya García, María José, Arévalo Mora, Cristina María, Vázquez Gámez, María de los Ángeles, Allain, Jean Paul, Torres Hernández, Yadir |
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| Přispěvatelé: | Universidad de Sevilla. Departamento de Citología e Histología Normal y Patológica, Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Universidad de Sevilla. Departamento de Medicina, European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER), Junta de Andalucía, Universidad Complutense de Madrid |
| Rok vydání: | 2020 |
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| Zdroj: | idUS: Depósito de Investigación de la Universidad de Sevilla Universidad de Sevilla (US) idUS. Depósito de Investigación de la Universidad de Sevilla instname |
| Popis: | A great variety of powder metallurgy techniques can produce biomimetic porous titanium structures with similar mechanical properties to host bone tissue. In this work, loose sintering and space holder techniques, two frequently used metallurgical techniques, are compared to evaluate the influences of porosity (content, size, morphology and wall roughness), mechanical properties (stiffness and yield strength) and in-vitro cellular responses (adhesion and proliferation of myoblasts and osteoblasts). These comparisons are made to achieve the best balance between biomechanical and bifunctional behavior of a partial porous implant for cortical bone replacement. Cell adhesion (filopodia presence) and spreading were promoted on both porous surfaces and fully dense substrates (non-porous control surfaces). Porous scaffold samples designed using 50 vol.% NaCl space holder technique had an improved bioactive response over those obtained with the loose sintering technique due to higher roughness and scaffold pore diameter. However, the presence of large and heterogeneous pores compromises the mechanical reliability of the implant. Considering both scenarios, the substrates obtained with 40 vol.% NH4HCO3 and pore size ranges between 100 and 200 μm provide a balanced optimization of size and strength to promote in-vitro osseointegration. FEDER US-1259771 University Complutense of Madrid (UCM) C2C12-GFP Junta de Andalucía US-1259771 |
| Databáze: | OpenAIRE |
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