Interspecies conservation of organisation and function between nonhomologous regional centromeres

Autor: Tong, P, Pidoux, A, Toda, N, Ard, R, Berger, H, Shukla, M, Torres-Garcia, J, Müller, C, Nieduszynski, C, Allshire, R
Přispěvatelé: Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Otto-von-Guericke-Universität Magdeburg, Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Center for Pneumology, Donaustauf Hospital, Centre for Genetics and Genomics, University of Nottingham, UK (UON)
Rok vydání: 2019
Předmět:
Zdroj: Nature Communications
Nature Communications, Nature Publishing Group, 2019, 10 (1), pp.(2019) 10:2343. ⟨10.1038/s41467-019-09824-4⟩
Tong, P, Pidoux, A, Toda, N R T, Ard, R, Berger, H, Shukla, M, Torres-Garcia, J, Mueller, C A, Nieduszynski, C A & Allshire, R C 2019, ' Interspecies conservation of organisation and function between nonhomologous regional centromeres ', Nature Communications, vol. 10, 2343 . https://doi.org/10.1038/s41467-019-09824-4
University of Copenhagen
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
ISSN: 2041-1723
DOI: 10.1038/s41467-019-09824-4⟩
Popis: Despite the conserved essential function of centromeres, centromeric DNA itself is not conserved. The histone-H3 variant, CENP-A, is the epigenetic mark that specifies centromere identity. Paradoxically, CENP-A normally assembles on particular sequences at specific genomic locations. To gain insight into the specification of complex centromeres, here we take an evolutionary approach, fully assembling genomes and centromeres of related fission yeasts.Centromere domain organization, but not sequence, is conserved between Schizosaccharomyces pombe, S. octosporus and S. cryophilus with a central CENP-ACnp1 domain flanked by heterochromatic outer-repeat regions. Conserved syntenic clusters of tRNA genes and 5S-rRNA genes occur across the centromeres of S. octosporus and S. cryophilus, suggesting conserved function. Interestingly, nonhomologous centromere central-core sequences from S. octosporus and S. cryophilus are recognized in S. pombe, resulting in crossspecies establishment of CENP-ACnp1 chromatin and functional kinetochores. Therefore, despite the lack of sequence conservation, Schizosaccharomyces centromere DNA possesses intrinsic conserved properties that promote assembly of CENP-A chromatin.
Databáze: OpenAIRE
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