Effect of 2,4-D concentration in somatic embryos formation of cuban soybeans varieties
| Autor: | Idalmis Bermúdez-Caraballoso, Teresa Del Socorro Blanco, Pérez-Pérez, Jorge L., García, Lourdes R., Novisel Veitía, Raúl Collado, Damaris Torres, Carlos Romero |
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| Zdroj: | BASE-Bielefeld Academic Search Engine Biotecnología Vegetal, Vol 12, Iss 2 (2012) |
| Popis: | Developing new breeding programs of soybean crops needs an efficient systems for plants regeneration. Eventhough due this is impossible taking into account its genotype dependent condition. This study aimed to: determine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration in the formation of somatic embryos in soybean Cuban varieties (Incasoy-1, Incasoy-24, Incasoy-27 and lncasoy-35). .mmature cotyledons were used as plant material. Three treatmentswere established according tothe concentrations of 2,4-D (30, 40 and 50 mg l1). The number of somatic embryos per immature cotyledon were evaluated after 30 days. The necessary concentration of 2,4-D to obtain the mayor formation of embryos depended on the genotypes (30 mg l1 for Incasoy-24; 40 mg l- for lncasoy-27 and lncasoy-35; 40 and 50 mg M for rIcasoy-1). Dependdng oo the results obtained the concentration 40 mg í1 forthe cuban varieties was selected The lncasoy-24 was the exception since it responded betterwith a concentration of 30 mg r1. These results favorthe development of new programs for genetic breedig of soybeans in Cuba. Keywords: somatic embryogenesis, Glycine max, plants regeneration |
| Databáze: | OpenAIRE |
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