Measuring concentration fields in microfluidic channels in situ with a Fabry-Perot interferometer
| Autor: | Vogus, Douglas R, Mansard, Vincent, Rapp, Michael V, Squires, Todd M |
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| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Lab on a chip, vol 15, iss 7 Vogus, DR; Mansard, V; Rapp, MV; & Squires, TM. (2015). Measuring concentration fields in microfluidic channels in situ with a Fabry-Perot interferometer. Lab on a Chip, 15(7), 1689-1696. doi: 10.1039/c5lc00095e. UC Santa Barbara: Retrieved from: http://www.escholarship.org/uc/item/9k64z3hf |
| DOI: | 10.1039/c5lc00095e. |
| Popis: | © The Royal Society of Chemistry 2015. Recent advancements in microfluidic technology have allowed for the generation and control of complex chemical gradients; however, few general techniques can measure these spatio-temporal concentration profiles without fluorescent labeling. Here we describe a Fabry-Perot interferometric technique, capable of measuring concentration profiles in situ, without any chemical label, by tracking Fringes of Equal Chromatic Order (FECO). The technique has a sensitivity of 10-5RIU, which can be used to track local solute changes of ∼0.05% (w/w). The technique is spatially resolved (1 μm) and easily measures evolving concentration fields with ∼20 Hz rate. Here, we demonstrate by measuring the binary diffusion coefficients of various solutes and solvents (and their concentration-dependence) in both free solution and in polyethylene glycol diacrylate (PEG-DA) hydrogels. |
| Databáze: | OpenAIRE |
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