Bile-induced peptidoglycan remodelling in Salmonella enterica

Autor: Hernández Piñero, Sara Belén, Cava, Felipe, Pucciarelli, María Graciela, García del Portillo, Francisco, Pedro, Miguel Ángel de, Casadesús Pursals, Josep
Přispěvatelé: Universidad Autónoma de Madrid, Umeå University, Knut and Alice Wallenberg Foundation, Swedish Research Council, Ministerio de Economía y Competitividad (España), Junta de Andalucía, Universidad de Sevilla. Departamento de Genética
Rok vydání: 2015
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
idUS. Depósito de Investigación de la Universidad de Sevilla
ISSN: 1462-2920
Popis: Changes in the peptidoglycan (PG) structure of Salmonella enterica are detected in the presence of a sublethal concentration of sodium deoxycholate (DOC): (i) lower proportions of Braun lipoprotein (Lpp)-bound muropeptides; (ii) reduced levels of muropeptides cross-linked by L(meso)-diaminopimelyl-D(meso)-diaminopimelic acid (L-D) peptide bridges (3-3 cross-links). Similar structural changes are found in S.enterica cultures adapted to grow in the presence of a lethal concentration of DOC, suggesting that reduced anchoring of Braun protein to PG and low occurrence of 3-3 cross-links may increase S.enterica resistance to bile. This view is further supported by additional observations: (i) A triple mutant lacking L,D-transpeptidases YbiS, ErfK, and YcfS, which does not contain Lpp anchored to PG, is hyper-resistant to bile; (ii) enhanced 3-3 cross-linking upon overexpression of YnhG transpeptidase causes a decrease in bile resistance. These observations suggest that remodelling of the cell wall may be added to the list of adaptive responses that permit survival of S.enterica in the presence of bile.
This study was supported by Grants BIO2010-15023 (to JC) and BIO2010-18885 (to FGP) from the Spanish Ministerio de Economía y Competitividad (MINECO) and the European Regional Fund, Grant CSD2008-00013 from the Consolider-Ingenio 2010 Program of MINECO (to JC and FGP), and Grant CVI-5879 from the Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía (to JC). Research in the Cava lab is supported by MINECO (RYC-2010-06241), Universidad Autónoma de Madrid (UAM-38), Laboratory for Molecular Infection Medicine Sweden (MIMS), Knut and Alice Wallenberg Foundation (KAW) and the Swedish Research Council.
Databáze: OpenAIRE
Externí odkaz: