Study of rumen bacterial and anaerobic fungal population level by TRFLP and ARISA; effect of the protein level and type of carbohydrate
| Autor: | Belanche, Alejandro, Pinloche, Eric, Edwards, Joan, Moorby, Jon, Doreau, Michel, Newbold, Jamie |
|---|---|
| Přispěvatelé: | Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Unité de Recherches sur les Herbivores (URH), Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | 41. Jornadas de Estudio y XIV Jornadas sobre Producción Animal 41. Jornadas de Estudio y XIV Jornadas sobre Producción Animal, May 2011, Zaragoza, España |
| Popis: | International audience; Four rumen-cannulated dairy cows were fed with two levels of degradable protein (110 vs. 80% of requirements) and two type of carbohydrates (NDF/starch of 2.8 and 1.3) according to a 4x4 Latin square. Rumen contents were sampled at 0, 2.5 and 5h after feeding for DNA extraction. Bacterial and anaerobic fungal populations were studied by using TRFLP and ARISA respectively. Sampling time modified neither the microbial population structure nor the biodiversity indexes. Bacterial population composition was affected by the type of carbohydrate used but did not modify the bacterial diversity, suggesting a shift in the bacterial species profile but the maintenance of population structure. Bacterial clustering was also affected by the protein level, particularly in animals upplemented with starch. Anaerobic fungal population was also affected by the type of carbohydrate, and fibrous diet increased the number of amplicons (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|