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Cancer is a major public health problem, young cancer patients therefore undergo chemotherapy, and most of them may lose their fertility. DNA damage level provides important clues about the quality and reproductive potential of spermatozoa. In this study, we evaluated the levels of both DNA fragmentation and abnormal DNA integrity in the epididymal sperms of New Zealand rabbit (thyctolagus cuniculus) after cryopreservation using the terminal deoxyribonucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) assay and the toluidine blue (TB) staining methods and assessed the effects of paclitaxel, resveratrol, L-glutamine (LG), and basal medium eagle (BME) solution on DNA damage. Paclitaxel induced the levels of both DNA damages in the sperms, but resveratrol ameliorated this effect. LG and BME supplementation to the extender prevented the sperm samples from DNA fragmentation after cryopreservation. Chemotherapy drugs containing paclitaxel can cause the sperm DNA to be damaged, and hence adversely affect the fertility of male cancer patients of reproductive age. The administration of resveratrol together with paclitaxel may ameliorate the DNA damage inducing effect of paclitaxel. Sperm banking and cryopreservation with the appropriate cryoprotectants such as LG and BME prior to cancer treatment can also be suggested to all male cancer patients of reproductive age facing cancer treatment for fertility preservation. (C) 2019 Elsevier Inc. All rights reserved. C1 [Ili, Pinar] Pamukkale Univ, Denizli Vocat Sch Hlth Serv, Dept Med Serv & Tech, Denizli, Turkey. [Sari, Fikret] Pamukkale Univ, Tavas Vocat Sch, Dept Plant & Anim Prod, Denizli, Turkey. [Bucak, Mustafa Numan; Ataman, Mehmet Bozkurt] Selcuk Univ, Fac Vet Med, Dept Reprod & Artificial Inseminat, Konya, Turkey. [Orturk, Caner] Aksaray Univ, Fac Vet Med, Dept Reprod & Artificial Inseminat, Aksaray, Turkey. [Gungor, Sukru] Mehmet Akif Ersoy Univ, Fac Vet Med, Dept Reprod & Artificial Inseminat, Burdur, Turkey. |
