Development of Gold nanoparticles for the characterization of different bacteria by Surface Enhanced Raman Scattering

Autor: Elie Akanny, Anne Bonhommé, Laurence Bois, Carine Commun, Anne Doleans-Jordheims, François Bessueille, Sandrine Bourgeois, Claire Bordes
Přispěvatelé: Chemometrics and Theoretical Chemistry - Chimiométrie et chimie théorique, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), spectroscopies vibrationnelles, Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Laboratoire de Bactériologie, hospices civils de Lyon, Surfaces, Laboratoire d'automatique, de génie des procédés et de génie pharmaceutique (LAGEPP), Université de Lyon-Université de Lyon-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), co-organisée par le Groupe Formulation de la Société Chimique de France (SCF) et l'Association de Pharmacie Galénique Industrielle (APGI) via le Laboratoire d’Automatique et de Génie des Procédés (LAGEPP)
Předmět:
Zdroj: HAL
Journées de Formulation 2019 : “Avancées dans la formulation de substances actives”
Journées de Formulation 2019 : “Avancées dans la formulation de substances actives”, Jan 2019, Lyon, France
Popis: National audience; Identification and quantification of microorganisms has become an essential issue in many areas like human health care and diagnostics, food and water safety and biodefense [1]. The conventional counting method, using plating and culturing, is the most straightforward and widely used method for this purpose but it appears tedious and time consuming. Since its discovery, Surface Enhanced Raman Spectroscopy (SERS) technique is increasingly used as faster and more convenient alternative. This technique allows to provide a high enhancement of Raman scattering from molecules adsorbed on a rough noble metal surface (silver, gold or copper). This phenomenon is explained by the combination of two well documented mechanisms: Electromagnetic effect (EE) and chemical effect (CE) [2]. The objective of our work was the development of gold nanoparticles (AuNps) as SERS substrate for the characterization of bacteria in order to avoid the use of the tiresome conventional counting method.The present study was divided in two parts. The first part consisted in developing the AuNPs synthesis method. Physico-chemical features of the synthetized AuNPs and their SERS efficiency using R6G model analyte have been investigated as a function of the synthesis experimental conditions. In the second part, the optimal operating conditions were used for bacteria characterization. The developed method was first tested for the quantification of Lactobacillus rhamnosus GG (LGG), a Gram-positive bacterium indicated for the treatment or the prevention of antibiotic-associated diarrhea. This strain being widely used in encapsulation systems, its characterization is required to determine encapsulation efficiency and bacteria release kinetics [3]. Then the potential of the developed method was investigated by characterizing other bacterial strains such as gram- negative bacterium Escherichia coli and gram-positive bacterium Bacillus subtilis.
Databáze: OpenAIRE
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