SARS-CoV-2 surveillance for a non-human primate breeding research facility
| Autor: | Yee, JoAnn L, Van Rompay, Koen KA, Carpenter, Amanda B, Nham, Peter B, Halley, Bryson M, Iyer, Smita S, Hartigan-O'Connor, Dennis J, Miller, Christopher J, Roberts, Jeffrey A |
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| Rok vydání: | 2020 |
| Předmět: |
testing algorithm
Antibodies Vaccine Related Feces Betacoronavirus antibody Biodefense Virology Animals Humans management practices Viral Veterinary Sciences Pandemics Lung SARS-CoV-2 Prevention Monkey Diseases COVID-19 Pneumonia Macaca mulatta Infectious Diseases Emerging Infectious Diseases Good Health and Well Being RNA PCR RNA Coronavirus Infections Infection Sentinel Surveillance Zoology Biotechnology |
| Zdroj: | Journal of medical primatology, vol 49, iss 6 |
| Popis: | BackgroundThe emergence of SARS-CoV-2 and the ensuing COVID-19 pandemic prompted the need for a surveillance program to determine the viral status of the California National Primate Research Center non-human primate breeding colony, both for reasons of maintaining colony health and minimizing the risk of interference in COVID-19 and other research studies.MethodsWe collected biological samples from 10% of the rhesus macaque population for systematic testing to detect SARS-CoV-2 virus by RT-PCR and host antibody response by ELISA. Testing required the development and validation of new assays and an algorithm using in laboratory-developed and commercially available reagents and protocols.Results and conclusionsNo SARS-CoV-2 RNA or antibody was detected in this study; therefore, we have proposed a modified testing algorithm for sentinel surveillance to monitor for any future transmissions. As additional reagents and controls become available, assay development and validation will continue, leading to the enhanced sensitivity, specificity, accuracy, and efficiency of testing. |
| Databáze: | OpenAIRE |
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