Discrepancy between molecular structure and ligand selectivity of a testicular follicle-stimulating hormone receptor of the African catfish (Clarias gariepinus)
| Autor: | Bogerd, J., Blomenröhr, M., Andersson, E., van der Putten, H., Tensen, C.P., Vischer, H F, Granneman, Joke C M, Janssen-Dommerholt, C, Goos, H.J., Schulz, Rüdiger W |
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| Přispěvatelé: | Medicinal chemistry, AIMMS |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Male
Embryo Nonmammalian Messenger Gene Expression Kidney Chorionic Gonadotropin Complementary Receptors FSH Testis Cloning Molecular Non-U.S. Gov't Catfishes Phylogeny Nonmammalian Research Support Non-U.S. Gov't Seminal Vesicles Recombinant Proteins Embryo Androgens Receptors FSH Female hormones hormone substitutes and hormone antagonists endocrine system animal structures DNA Complementary Inositol Phosphates Molecular Sequence Data Research Support Transfection Cell Line Species Specificity Journal Article Animals Humans Amino Acid Sequence RNA Messenger SDG 14 - Life Below Water Base Sequence Mammalian fungi Ovary Molecular DNA Luteinizing Hormone Embryo Mammalian RNA Follicle Stimulating Hormone Sequence Alignment Cloning |
| Zdroj: | Bogerd, J, Blomenröhr, M, Andersson, E, van der Putten, H, Tensen, C P, Vischer, H F, Granneman, J C M, Janssen-Dommerholt, C, Goos, H J & Schulz, R W 2001, ' Discrepancy between molecular structure and ligand selectivity of a testicular follicle-stimulating hormone receptor of the African catfish (Clarias gariepinus) ', Biology of Reproduction, vol. 64, no. 6, pp. 1633-43 . https://doi.org/10.1095/biolreprod64.6.1633 Biology of Reproduction, 64(6), 1633-43. Society for the Study of Reproduction |
| ISSN: | 0006-3363 |
| DOI: | 10.1095/biolreprod64.6.1633 |
| Popis: | A putative FSH receptor (FSH-R) cDNA was cloned from African catfish testis. Alignment of the deduced amino acid sequence with other (putative) glycoprotein hormone receptors and analysis of the African catfish gene indicated that the cloned receptor belonged to the FSH receptor subfamily. Catfish FSH-R (cfFSH-R) mRNA expression was observed in testis and ovary; abundant mRNA expression was also detected in seminal vesicles. The isolated cDNA encoded a functional receptor since its transient expression in human embryonic kidney (HEK-T) 293 cells resulted in ligand-dependent cAMP production. Remarkably, African catfish LH (cfLH; the catfish FSH-like gonadotropin has not been purified yet) had the highest potency in this system. From the other ligands tested, only human recombinant FSH (hrFSH) was active, showing a fourfold lower potency than cfLH, while hCG and human TSH (hTSH) were inactive. Human CG (as well as cfLH, hrFSH, eCG, but not hTSH) stimulated testicular androgen secretion in vitro but seemed to be unable to bind to the cfFSH-R. However, it was known that hCG is biologically active in African catfish (e.g., induction of ovulation). This indicated that an LH receptor is also expressed in African catfish testis. We conclude that we have cloned a cDNA encoding a functional FSH-R from African catfish testis. The cfFSH-R appears to be less discriminatory for its species-specific LH than its avian and mammalian counterparts. |
| Databáze: | OpenAIRE |
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