Hypertrophic cardiomyopathy β-cardiac myosin mutation (P710R) leads to hypercontractility by disrupting super relaxed state
| Autor: | Vander Roest, Alison Schroer, Liu, Chao, Morck, Makenna M, Kooiker, Kristina Bezold, Jung, Gwanghyun, Song, Dan, Dawood, Aminah, Jhingran, Arnav, Pardon, Gaspard, Ranjbarvaziri, Sara, Fajardo, Giovanni, Zhao, Mingming, Campbell, Kenneth S, Pruitt, Beth L, Spudich, James A, Ruppel, Kathleen M, Bernstein, Daniel |
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| Rok vydání: | 2021 |
| Předmět: |
Cardiomyopathy
1.1 Normal biological development and functioning Induced Pluripotent Stem Cells Bioengineering Cardiovascular Cell Line Ventricular Myosins Mice Myofibrils Models Genetics beta-cardiac myosin Animals Humans 2.1 Biological and endogenous factors Genetic Predisposition to Disease Cell Size Myocytes Stem Cell Research - Induced Pluripotent Stem Cell - Human Stem Cell Research - Induced Pluripotent Stem Cell hiPSC-CMs Biological Stem Cell Research hypertrophic cardiomyopathy Myocardial Contraction Actins β-cardiac myosin Biomechanical Phenomena Heart Disease super relaxed state Hypertrophic Mutation optical trapping Calcium Cardiac |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America, vol 118, iss 24 |
| Popis: | Hypertrophic cardiomyopathy (HCM) is the most common inherited form of heart disease, associated with over 1,000 mutations, many in β-cardiac myosin (MYH7). Molecular studies of myosin with different HCM mutations have revealed a diversity of effects on ATPase and load-sensitive rate of detachment from actin. It has been difficult to predict how such diverse molecular effects combine to influence forces at the cellular level and further influence cellular phenotypes. This study focused on the P710R mutation that dramatically decreased in vitro motility velocity and actin-activated ATPase, in contrast to other MYH7 mutations. Optical trap measurements of single myosin molecules revealed that this mutation reduced the step size of the myosin motor and the load sensitivity of the actin detachment rate. Conversely, this mutation destabilized the super relaxed state in longer, two-headed myosin constructs, freeing more heads to generate force. Micropatterned human induced pluripotent derived stem cell (hiPSC)-cardiomyocytes CRISPR-edited with the P710R mutation produced significantly increased force (measured by traction force microscopy) compared with isogenic control cells. The P710R mutation also caused cardiomyocyte hypertrophy and cytoskeletal remodeling as measured by immunostaining and electron microscopy. Cellular hypertrophy was prevented in the P710R cells by inhibition of ERK or Akt. Finally, we used a computational model that integrated the measured molecular changes to predict the measured traction forces. These results confirm a key role for regulation of the super relaxed state in driving hypercontractility in HCM with the P710R mutation and demonstrate the value of a multiscale approach in revealing key mechanisms of disease. |
| Databáze: | OpenAIRE |
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