Recombinant LPG3 Stimulates IFN-Γ and TNF-Α Secretion by Human NK Cells
| Autor: | Rasolzadeh, S., Haji Fatahaliha, M., Hosseini, M., Jafari, R., Miahipour, A., Movassaghpour, A. A., Babalo, Z., Sima Rafati, Yousefi, M. |
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| Zdroj: | Europe PubMed Central Scopus-Elsevier Iranian Journal of Parasitology, Vol 10, Iss 3 (2015) Iranian Journal of Parasitology, Vol 10, Iss 4 (2015) |
| Popis: | Background: Natural killer (NK) cells play an important role in early stages of innate immune responses against viral and tumoral attacks. Activation of NK cells by leishmaniasis results in secretion of cytokines such as interferon (IFN)-γ and tumor necrosis factor (TNF)-α, which enhances the phagocytosis and clearance of parasite. Lipophosphoglycan 3 (LPG3), the Leishmania homologous with GRP94 (glucose regulated protein 94), a member of HSP90 family, contributes to LPG assembly as the most abundant macromolecule on the surface of Leishmania promastigotes. Methods: We purified NK cells from healthy individuals (n=10) using magnetic-activated cell sorting (MACS) technology. Purified NK cells were co-incubated with different concentrations of recombinant LPG3 (rLPG3), and its N-terminal (NT) and C-terminal (CT) fragments. Finally, the production of IFN-γ and TNF-α by NK cells were measured by ELISA. Results: Recombinant LPG3 but not its fragments (CT and NT), can significantly enhance the production of TNF-α by NK cells (P |
| Databáze: | OpenAIRE |
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