| Autor: |
Birtele, Marcella, Fiorenzano, Alessandro, Nelander, Jenny, Parmar, Malin |
| Rok vydání: |
2019 |
| Předmět: |
|
| DOI: |
10.5281/zenodo.3358629 |
| Popis: |
Parkinson disease (PD) is characterized by a loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc). The selective loss of this cell population makes PD a good candidate for cell-based therapies. Clinical trials using cells derived from human fetal ventral midbrain (hVM) have shown that dopamine release was restored to normal levels and in some PD patients produced substantial long-term clinical improvement. Although new sources of cells such as human embryonic stem cells (hESCs) and pluripotent stem cells (IPCs) are now been the focus of studies and new clinical trials, the hVM tissue represent the gold standard for cell based therapies and serve as important comparator for new cell sources. Therefore there is a need of an increased understanding and characterization of this tissue with new technologies such as organoid culture conditions and single cell sequencing. This study is designed to give a detailed characterization of human fetal VM tissue in standard culture conditions (2D) versus organoids (3D). We used immunocytochemistry to study the presence of ventral midbrain markers in 2D versus 3D condition together with single cell RNA sequencing to transcriptionally compare fetal VM tissue in standard or organoid cultures. Our analysis confirmed that the 3D condition preserve the neuronal composition of the tissue more than standard culture where instead, over a long period of time, the majority of the cells are non-neuronal. This suggest that 3D culture represent a better condition to maintain and study dopaminergic neurons in culture. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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