Sığır Serum Albumini İlave Edilmiş Lesitin Bazlı Sulandırıcı İle Sulandırılan Koç Spermasının Dondurma-Çözdürme Sonrası Uzun Süreli İnkübasyon Direnci]
| Autor: | Alcay, Selim, Toker, Mehmed Berk, Gökçe, Elif, Önder, Nail Tekin, Üstüner, Burcu, Nur, Zekariya |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Cryopreservation
Liquid Storage Soybean Lecithin Veterinary medicine Motility incubation resilience Incubation resilience cryopreservation Egg-Yolk Quality Spermatozoa Antioxidants Ram spermatozoa Dna Fragmentation Bovine serum albumin bovine serum albumin SF600-1100 Sperm Capacitation ram spermatozoa |
| Zdroj: | Kafkas Universitesi Veteriner Fakultesi Dergisi, Vol 25, Iss 3, Pp 291-297 (2019) |
| ISSN: | 1309-2251 1300-6045 |
| Popis: | The objective of the study was to determine the optimum concentration of BSA in lecithin-based extender for post-thawing quality and incubation resilience (0 h, 6 h and 10 h) of ram sperm. Ejaculates were collected from five rams via electro ejaculation. Ejaculates were mixed to obtain pooled semen.Then, pooled semen was diluted with soybean lecithin-based extender without BSA (control) or supplemented with different concentrations of BSA (2.5 mg/mL, 5 mg/mL, 7.5 mg/mL and 10 mg/mL), at a final concentration of 150x106 spermatozoon/mL. Sperm motility, plasma membrane functional integrity (HOST), mitochondrial activity (rhodamine123), capacitation status (CTC), and DNA integrity (TUNEL) were evaluated. At the 10 h incubation, motility, plasma membrane functional integrity and mitochondria! function were better preserved in the BSA5 group compared to the control group. It was determined that high doses of BSA (5 mg/mL, 7.5 mg/mL and 10 mg/mL) affected acrosome reaction.The highest acrosome reaction rates were obtained in BSA10 groups in 6 h and 10 h incubation (P |
| Databáze: | OpenAIRE |
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