In vitro propagation of critically endangered species Scilla autumnalis L. – biochemical analyses of the regenerants
| Autor: | Banciu, Cristian, Elena Monica Mitoi, Helepciuc, Florenta, Aldea, Florentina |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Analele Universitatii din Oradea: Fascicula Biologie, Vol TOM XVII, Iss 2, Pp 318-323 (2010) BASE-Bielefeld Academic Search Engine |
| ISSN: | 1844-7589 1224-5119 |
| Popis: | The present study belongs to the international efforts for plant conservation from the areas threatened by human activities. The saline soils areas are restricting for agriculture and in some cases for fishery facilities and the plant species are extinct from those areas. Scilla autumnalis L. is one of the threatened plants (rare on the national red list of vascular plants from Romania) that grows in the Natural Park Comana, Giurgiu County, South Romania. Seeds from plants grown in the natural habitat have been used for in vitro plant regeneration and multiplication. After successfully rooting and acclimatization of the regenerated plantlets from germinated seeds, biochemical studies have been performed in order to compare the regenerants from in vitro cultures with native plants from genetically point of view. Peroxydase and esterase’s spectra were the biochemical markers used.The results indicated that this plant species can be multiplicated, rooted and acclimatized on synthetic medium (MS supplemented with NAA, IBA, IAA, kinetin and BAP) with a good efficiency and the regenerants had no genetic alterations determinated by culture conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|