Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds

Autor: Rodrigues, José Ariévilo Gurgel, Barcellos, Priscila Gomes, Glauser, Bianca Fernandes, Quinderé, Ana Luíza Gomes, Benevides, Norma Maria Barros, Tovar, Ana Maria Freire, Mourão, Paulo Antônio de Souza
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Acta Scientiarum : Biological Sciences, Vol 40, Iss 1 (2018)
Acta Scientiarum. Biological Sciences; Vol 40 (2018): Publicação Contínua; e34463
Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e34463
Acta Scientiarum Biological Sciences
Universidade Estadual de Maringá (UEM)
instacron:UEM
ISSN: 1679-9283
1807-863X
Popis: The biotechnological value of macroalgae for screening assays of thrombin generation-TG using sulfated polysaccharides-SPs as substitutes to heparin has been poorly explored. Five Brazilian species of macroalgae (Gracilaria birdiae, Acanthophora muscoides, Halymenia sp., Caulerpa cupressoides and C. racemosa) were analyzed and compared for their abundance, physical-chemical characteristics and in vitro anticoagulant assays of activated partial thromboplastin time-APTT, prothrombin time-PT and TG. Papain extraction yielded (p < 0.001) from 0.66±0.03% (C. racemosa) to 41.60±1.10% (Halymenia sp.) of crude SPs varying sulfate (8.41-42.60%) and total sugars (47.80-70.53%). Crude SPs showed difference in mobility and resolution pattern by agarose electrophoresis, while polyacrylamide analysis revealed SPs of > 100 kDa. These procedures, combined with the use of Stains-All, also indicated nonSPs. APTTs ranged from 2.81 (A. muscoides) to 21.30 IU (Halymenia sp.) vs. heparin (193 IU), and were dependent on sulfation of the crude SPs. PT was not altered. With respect to TG assay, crude SPs modified concentration-dependent and independently from molecular mass TG by both intrinsic/extrinsic pathways in 60-fold diluted human plasma, with total intrinsic inactivation using crude SPs from A. muscoides in parallel to heparin (p < 0.05). Thrombosis in vitro is differentially modulated by distinct crude SPs from Brazilian seaweeds.
Databáze: OpenAIRE
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