Additively manufactured carbon/black-integrated polylactic acid 3Dprintedsensor for simultaneous quantification of uric acid and zinc in sweat

Autor: Siervo, Abner de, 1972
Přispěvatelé: UNIVERSIDADE ESTADUAL DE CAMPINAS
Rok vydání: 2021
Předmět:
Zdroj: Repositório Institucional da Unicamp
Universidade Estadual de Campinas (UNICAMP)
instacron:UNICAMP
Repositório da Produção Científica e Intelectual da Unicamp
ISSN: 7172-2017
Popis: Agradecimentos: This research was supported by Sao Paulo Research Foundation (FAPESP) (Grant numbers: 2007/08244-5, 2007/54829-5, 2014/50867-3, 2017/18574-4, 2018/14462-0, and 2020/00325-0), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (Pró-Forenses Edital 25/2014) and Conselho Nacional de Desenvolvimento Científco e Tecnológico (CNPq) (Grant Number: 305605/2017-8, 307172-2017-0, 311847/2018-8, 465389/2014-7 - INCTBio and 302839/2020-8) Abstract: For the first time the development of an electrochemical method for simultaneous quantification of Zn2+ and uric acid (UA) in sweat is described using an electrochemically treated 3D-printed working electrode. Sweat analysis can provide important information about metabolites that are valuable indicators of biological processes. Improved performance of the 3D-printed electrode was achieved after electrochemical treatment of its surface in an alkaline medium. This treatment promotes the PLA removal (insulating layer) and exposes carbon black (CB) conductive sites. The pH and the square-wave anodic stripping voltammetry technique were carefully adjusted to optimize the method. The peaks for Zn2+ and UA were well-defined at around - 1.1 V and + 0.45 V (vs. CB/PLA pseudo-reference), respectively, using the treated surface under optimized conditions. The calibration curve showed a linear range of 1 to 70 mu g L-1 and 1 to 70 mu mol L-1 for Zn2+ and UA, respectively. Relative standard deviation values were estimated as 4.8% (n = 10, 30 mu g L-1) and 6.1% (n = 10, 30 mu mol L-1) for Zn2+ and UA, respectively. The detection limits for Zn2+ and UA were 0.10 mu g L-1 and 0.28 mu mol L-1, respectively. Both species were determined simultaneously in real sweat samples, and the achieved recovery percentages were between 95 and 106% for Zn2+ and 82 and 108% for UA FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ Fechado
Databáze: OpenAIRE
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