Quantitative volumetric Raman imaging of three dimensional cell cultures
| Autor: | Kallepitis, Charalambos, Bergholt, Mads S., Mazo, Manuel M., Leonardo, Vincent, Skaalure, Stacey C., Maynard, Stephanie A., Stevens, Molly M. |
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| Přispěvatelé: | Medical Research Council (MRC), Kaust, Wellcome Trust, Commission of the European Communities |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Nature Communications, Vol 8, Iss 1, Pp 1-9 (2017) Nature Communications Kallepitis, C, Bergholt, M S, Mazo, M M, Leonardo, V, Skaalure, S C, Maynard, S A & Stevens, M M 2017, ' Quantitative volumetric Raman imaging of three dimensional cell cultures ', Nature Communications, vol. 8, no. 1, 14843 . https://doi.org/10.1038/ncomms14843 |
| ISSN: | 2041-1723 |
| Popis: | The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell-material interactions. Here, we present a computational framework for label-free quantitative volumetric Raman imaging (qVRI). We apply qVRI to a selection of biological systems: human pluripotent stem cells with their cardiac derivatives, monocytes and monocyte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomimetic hydrogels that supplied a 3D cell culture environment. We demonstrate visualization and quantification of fine details in cell shape, cytoplasm, nucleus, lipid bodies and cytoskeletal structures in 3D with unprecedented biomolecular specificity for vibrational microspectroscopy. |
| Databáze: | OpenAIRE |
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