Deregulation of microRNA expression in monocytes and CD4+ T lymphocytes from patients with axial spondyloarthritis

Autor: Fogel, Olivier, Bugge Tinggaard, Andreas, Fagny, Maud, Sigrist, Nelly, Roche, Elodie, Leclere, Laurence, Deleuze, Jean-François, Batteux, Frederic, Dougados, Maxime, Miceli-Richard, Corinne, Tost, Jörg
Přispěvatelé: Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Service de rhumatologie [CHU Cochin], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Descartes - Paris 5 (UPD5), Aarhus University [Aarhus], Hôpital Cochin [AP-HP], Immunorégulation - Immunoregulation, Institut Pasteur [Paris] (IP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Cité (UPCité), The research described in this manuscript was mainly funded by a Sirius research award (UCB Pharma S.A.). Additional funding was obtained through a Passerelle research award (Pfizer), iCARE (MSD Avenir) and the institutional budget of the CNRGH. O.F. is the recipient of a PhD fellowship from Assistance Publique - Hopitaux de Paris / CEA (poste accueil AP-HP)., Fagny, Maud
Jazyk: angličtina
Rok vydání: 2019
Předmět:
MESH: Antirheumatic Agents
lcsh:Diseases of the musculoskeletal system
MESH: Spondylarthritis
[SDV]Life Sciences [q-bio]
MESH: Monocytes
Monocytes
MESH: Gene Expression Profiling
MESH: Reverse Transcriptase Polymerase Chain Reaction
Spondyloarthritis
MESH: Cohort Studies
[SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system
[SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM]
MESH: Humans
MESH: Middle Aged
CD4 T lymphocytes
MESH: CD4-Positive T-Lymphocytes
MESH: Adult
CD4+ T lymphocytes
[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM]
MESH: Male
[SDV] Life Sciences [q-bio]
[SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system
Epigenetics
lcsh:RC925-935
MiRNA
MESH: Spine
MESH: Female
MESH: MicroRNAs
MESH: Cells
Cultured
Zdroj: Arthritis Research & Therapy, Vol 21, Iss 1, Pp 1-14 (2019)
Fogel, O, Bugge Tinggaard, A, Fagny, M, Sigrist, N, Roche, E, Leclere, L, Deleuze, J F, Batteux, F, Dougados, M, Miceli-Richard, C & Tost, J 2019, ' Deregulation of microRNA expression in monocytes and CD4 + T lymphocytes from patients with axial spondyloarthritis ', Arthritis Research and Therapy, vol. 21, no. 1, 51 . https://doi.org/10.1186/s13075-019-1829-7
Arthritis Research & Therapy
Arthritis Research & Therapy, 2019, 21 (1), pp.1-14. ⟨10.1186/s13075-019-1829-7⟩
ISSN: 1478-6362
DOI: 10.1186/s13075-019-1829-7
Popis: BACKGROUND: MicroRNAs (MiRs) play an important role in the pathogenesis of chronic inflammatory diseases. This study is the first to investigate miR expression profiles in purified CD4+ T lymphocytes and CD14+ monocytes from patients with axial spondyloarthritis (axSpA) using a high-throughput qPCR approach. METHODS: A total of 81 axSpA patients fulfilling the 2009 ASAS classification criteria, and 55 controls were recruited from October 2014 to July 2017. CD14+ monocytes and CD4+ T lymphocytes were isolated from peripheral blood mononuclear cells. MiR expression was investigated by qPCR using the Exiqon Human MiRnome panel I analyzing 372 miRNAs. Differentially expressed miRNAs identified in the discovery cohort were validated in the replication cohort. RESULTS: We found a major difference in miR expression patterns between T lymphocytes and monocytes regardless of the patient or control status. Comparing disease-specific differentially expressed miRs, 13 miRs were found consistently deregulated in CD14+ cells in both cohorts with miR-361-3p, miR-223-3p, miR-484, and miR-16-5p being the most differentially expressed. In CD4+ T cells, 11 miRs were differentially expressed between patients and controls with miR-16-1-3p, miR-28-5p, miR-199a-5p, and miR-126-3p were the most strongly upregulated miRs among patients. These miRs are involved in disease relevant pathways such as inflammation, intestinal permeability or bone formation. Mir-146a-5p levels correlated inversely with the degree of inflammation in axSpA patients. CONCLUSIONS: We demonstrate a consistent deregulation of miRs in both monocytes and CD4+ T cells from axSpA patients, which could contribute to the pathophysiology of the disease with potential interest from a therapeutic perspective. ispartof: Arthritis Res Ther vol:21 issue:1 pages:51- ispartof: location:England status: Published online
Databáze: OpenAIRE
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