The origin of the self-compatible almond 'Supernova'

Autor: Bošković, Radovan I., Tobutt, Kenneth R., Marchese, Annalisa, Martínez-García, Pedro J.
Přispěvatelé: Università degli Studi di Palermo, Ministerio de Educación y Ciencia (España), Mount Trust, Department for Environment, Food & Rural Affairs (UK)
Rok vydání: 2008
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
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Popis: 3 pages, 1 figure, 1 table.
The almond cultivar 'Supernova' is reported in the literature as a late-flowering self-compatible mutant, obtained by the irradiation of the early-flowering self-incompatible cultivar 'Fascionello'. Our work to investigate the molecular basis of this form of self-compatibility has called into question the origin of 'Supernova'. Test selfing was performed both on 'Supernova' and on the accession of 'Fascionello' from which it was derived –'Fascionello-Rome'. Both proved self-compatible. Amplification of S-RNase alleles, using consensus primers from the signal peptide region to the second conserved region of the S-RNase gene and primers specific for allele Sf, confirmed 'Supernova' and 'Fascionello-Rome' indeed have the same (in)compatibility genotype S1Sf as the self-compatible cultivar 'Tuono'. Nine microsatellite markers were used to fingerprint the relevant accessions; 'Supernova' and 'Fascionello-Rome' were undistinguishable from 'Tuono'. Two Sicilian accessions, 'Fascionello' and 'Falso Fascionello', lacked Sf and differed at the SSR level, indicating no close relationship with 'Supernova'. Therefore we concluded that 'Fascionello-Rome' is actually the same as 'Tuono' and that 'Supernova' originates from 'Tuono' from which it derives its self-compatibility.
Annalisa Marchese acknowledges receipt of a grant from Palermo University, Pedro J. Martinez-Garcia a travel scholarship from the Spanish Ministry of Education and Science and Radovan Boskovic a grant from the Mount Trust. Prunus genetics at EMR is funded by Defra.
Databáze: OpenAIRE
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