| Autor: |
Scheer, Hélène, de Almeida, Caroline, Ferrier, Emilie, Poirier, Laure, Pflieger, David, Sement, François, Koechler, Sandrine, Piermaria, Christina, Chicher, Johana, Kuhn, Lauriane, Hammann, Philippe, Zuber, Hélène, GAGLIARDI, Dominique |
| Přispěvatelé: |
Institut de biologie moléculaire des plantes (IBMP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Plateforme Protéomique Strasbourg - Esplanade (IBMC / CNRS FRC1589 / UNIV Strasbourg), Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Moléculaire et Cellulaire [Strasbourg] (IBMC), ANR-15-CE12-0008,3'modRN,Modifications des extrémités 3' des ARNm par addition de nucléotides: impact sur la traduction et la dégradation des ARNm chez Arabidopsis thaliana(2015), ANR: NetRNA,ANR-10-LABX-0036_NETRNA |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Popis: |
Uridylation is a widespread modification destabilizing eukaryotic mRNAs. Yet, molecular mechanisms underlying TUTase-mediated mRNA degradation remain mostly unresolved. Here, we report that the Arabidopsis TUTase URT1 participates in a molecular network connecting several translational repressors/decapping activators including DECAPPING 5 (DCP5), the Arabidopsis ortholog of human LSM14 and yeast Scd6. A conserved Helical Leucine-rich Motif (HLM) within an intrinsically disordered region of URT1 binds to the LSm domain of DCP5. This interaction connects URT1 to additional decay factors like DDX6/Dhh1-like RNA helicases. The combination of in planta and in vitro analyses supports a model that explains how URT1 reduces the accumulation of oligo(A)-tailed mRNAs: first, by connecting decapping factors and second, because 3’ terminal uridines can intrinsically hinder deadenylation. Importantly, preventing the accumulation of excessively deadenylated mRNAs in Arabidopsis avoids the biogenesis of illegitimate siRNAs that silence endogenous mRNAs and perturb plant growth and development. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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