| Autor: |
Brahmbhatt, S, Hussain, R, Zafar, S, Dawood, G, Ottenhoff, THM, Drijfhout, JW, Bothamley, G, Smith, S, Lopez, FV, Dockrell, HM |
| Jazyk: |
angličtina |
| Rok vydání: |
2002 |
| ISSN: |
0009-9104 |
| Popis: |
In order to identify T cell epitopes within the Mycobacterium leprae 45-kD serine-rich antigen, we analysed responses to overlapping 17-mer peptides encompassing the whole antigen in non-exposed UK controls, Pakistani leprosy patients and tuberculosis patients in both the United Kingdom and Pakistan. This antigen has been described as M. leprae-specific, although it has a hypothetical homologue in M. tuberculosis. Human peripheral blood mononuclear cells were stimulated with peptide for 5 days and IFN-gamma measured in supernatants by ELISA. Some peptides were recognized more frequently by T cells from tuberculoid leprosy patients than those from UK controls, suggesting that such T cell epitopes might have diagnostic potential, while other peptides induced greater responses among UK control subjects. Short-term cell lines confirmed that these assays detected specific T cell recognition of these peptides. However, many tuberculosis patients also recognized these potentially specific peptides suggesting that there could be a true homologue present in M. tuberculosis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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