| Autor: |
Orange, Sam, Jordan, Alastair, Odell, Adam, Kavanagh, Owen, Hicks, Kirsty M., Eaglen, Tristan, Todryk, Stephen, Saxton, John M. |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| ISSN: |
0020-7136 |
| Popis: |
Epidemiological evidence shows that regular physical activity is associated with reduced risk of primary and recurrent colon cancer. However, the underlying mechanisms of action are poorly understood. We evaluated the effects of stimulating a human colon cancer cell line (LoVo) with human serum collected before and after an acute exercise bout versus non-exercise control serum on cancer cell proliferation. We also measured exercise-induced changes in\ud serum cytokines and intracellular protein expression to explore potential biological mechanisms. Blood samples were collected from 16 men with lifestyle risk factors for colon\ud cancer (age ≥50 years; body mass index ≥25 kg/m2; physically inactive) before and immediately after an acute bout of moderate-intensity aerobic interval exercise (6 x 5 min\ud intervals at 60% heart rate reserve) and a non-exercise control condition. Stimulating LoVo cells with serum obtained immediately after exercise reduced cancer cell proliferation compared with control (-5.7%; p=0.002). This was accompanied by a decrease in LoVo cell γ-H2AX expression (-24.6%; p=0.029), indicating a reduction in DNA damage. Acute exercise also increased serum IL-6 (24.6%, p=0.002). Furthermore, stimulating LoVo cells with recombinant IL-6 reduced γ-H2AX expression (β=-22.7%; p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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