| Autor: |
Tensen, Cornelius P, Cox, Kingsley J A, Smit, August B, van der Schors, Roel C, Meyerhof, Wolfgang, Richter, Dietmar, Planta, Rudi J, Hermann, Petra M, van Minnen, Jan, Geraerts, Wijnand P M, Knol, Jaco C, Burke, Julian F, Vreugdenhil, Erno, van Heerikhuizen, Harm |
| Jazyk: |
angličtina |
| Rok vydání: |
1998 |
| ISSN: |
0270-6474 |
| Popis: |
A novel G-protein–coupled receptor (GRL106) resembling neuropeptide Y and tachykinin receptors was cloned from the molluscLymnaea stagnalis. Application of a peptide extract from the Lymnaea brain to Xenopus oocytes expressing GRL106 activated a calcium-dependent chloride channel. Using this response as a bioassay, we purified the ligand for GRL106,Lymnaea cardioexcitatory peptide (LyCEP), an RFamide-type decapeptide (TPHWRPQGRF-NH2) displaying significant similarity to the Achatina cardioexcitatory peptide (ACEP-1) as well as to the recently identified family of mammalian prolactin-releasing peptides. In the Lymnaeabrain, the cells that produce egg-laying hormone are the predominant site of GRL106 gene expression and appear to be innervated by LyCEP-containing fibers. Indeed, LyCEP application transiently hyperpolarizes isolated egg-laying hormone cells. In theLymnaea pericardium, LyCEP-containing fibers end blindly at the pericardial lumen, and the heart is stimulated by LyCEPin vitro. These data confirm that LyCEP is an RFamide ligand for GRL106 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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