| Autor: |
Nowostawska, M., Corr, S.A., Byrne, S.J., Conroy, J., Volkov, Y., Gun'ko, Y.K. |
| Jazyk: |
angličtina |
| Rok vydání: |
2011 |
| ISSN: |
1477-3155 |
| Popis: |
Background: The use of silica coated magnetic nanoparticles as contrast agents has resulted in the production of\ud highly stable, non-toxic solutions that can be manipulated via an external magnetic field. As a result, the\ud interaction of these nanocomposites with cells is of vital importance in understanding their behaviour and\ud biocompatibility. Here we report the preparation, characterisation and potential application of new “two-in-one”\ud magnetic fluorescent nanocomposites composed of silica-coated magnetite nanoparticles covalently linked to a\ud porphyrin moiety.\ud \ud Method: The experiments were performed by administering porphyrin functionalised silica-coated magnetite\ud nanoparticles to THP-1 cells, a human acute monocytic leukaemia cell line. Cells were cultured in RPMI 1640\ud medium with 25 mM HEPES supplemented with heat-inactivated foetal bovine serum (FBS).\ud \ud Results: We have synthesised, characterised and analysed in vitro, a new multimodal (magnetic and fluorescent)\ud porphyrin magnetic nanoparticle composite (PMNC). Initial co-incubation experiments performed with THP-1\ud macrophage cells were promising; however the PMNC photobleached under confocal microscopy study. bmercaptoethanol (b-ME) was employed to counteract this problem and resulted not only in enhanced\ud fluorescence emission, but also allowed for elongated imaging and increased exposure times of the PMNC in a\ud cellular environment.\ud \ud Conclusion: Our experiments have demonstrated that b-ME visibly enhances the emission intensity. No deleterious\ud effects to the cells were witnessed upon co-incubation with b-ME alone and no increases in background\ud fluorescence were recorded. These results should present an interest for further development of in vitro biological\ud imaging techniques. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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