Studies with Chloroplast and Mitochondrial DNA I. Evidence of Sequence Homology between Chloroplast and Nuclear DNA (Broad Bean) and between Mitochondrial and Nuclear DNA (Rat Liver)

Autor: Kung, S.D., Moscarello, M.A., Williams, J.P.
Jazyk: angličtina
Předmět:
Zdroj: Biophysical Journal. (5):474-483
ISSN: 0006-3495
DOI: 10.1016/S0006-3495(72)86098-3
Popis: A mixture of broad bean chloroplast and nuclear DNA or rat liver mitochondrial and nuclear DNA was taken through a heating and annealing cycle, and examined by CsCl density gradient centrifugation. The formation of intermediates between the two DNAs during joint annealing was used as a method of detecting sequence homology in different DNA samples. Homology was found between chloroplast and nuclear DNA from broad bean and between mitochondrial and nuclear DNA from rat liver. Since this method produces only qualitative data no implication for possible nucleocytoplasmic relationship can be assessed.
Databáze: OpenAIRE