Evaluation of cytokine responses against novel Mtb antigens as diagnostic markers for TB disease

Autor: Awoniyi, DO, Teuchert, A, Sutherland, JS, Mayanja-Kizza, H, Howe, R, Mihret, A, Loxton, AG, Sheehama, J, Kassa, D, Crampin, AC, Dockrell, HM, Kidd, M, Rosenkrands, I, Geluk, A, Ottenhoff, TH, Corstjens, PL, Chegou, NN, Walzl, G, AE-TBC Consortium, COLLABORATORS, Kriel, M, van der Spuy, G, Stanley, K, Malherbe, S, Mcanda, S, Kriel, B, Phalane, KG, Essone, P, Owolabi, O, Sillah, A, Mendy, J, Gindeh, A, Donkor, S, Togun, T, Ota, M, Simukonda, F, Amberbir, A, Chilongo, F, Houben, R, Gebrezgeabher, A, Mesfin, G, Belay, Y, Gebremichael, G, Alemayehu, Y, van der Vyver, M, Amutenya, FN, Nelongo, JN, Monye, L, Sheehama, JA, Iipinge, S, Namuganga, AR, Muzanye, G, Nsereko, M, Peters, P, Bekele, Y, Tessema, B, Yamuah, L, Franken, K, Tjon Kon Fat, EM, de Dood, CJ, van der Ploeg-van Schip, JJ, Aagaard, C, Kaufmann, SH, Esterhuyse, MM, Cliff, JM
Jazyk: angličtina
Rok vydání: 2016
ISSN: 0163-4453
Popis: We investigated the accuracy of host markers detected in Mtb antigen-stimulated whole blood culture supernatant in the diagnosis of TB. : Prospectively, blood from 322 individuals with presumed TB disease from six African sites was stimulated with four different Mtb antigens (Rv0081, Rv1284, ESAT-6/CFP-10, and Rv2034) in a 24 h whole blood stimulation assay (WBA). The concentrations of 42 host markers in the supernatants were measured using the Luminex multiplex platform. Diagnostic biosignatures were investigated through the use of multivariate analysis techniques. : 17% of the participants were HIV infected, 106 had active TB disease and in 216 TB was excluded. Unstimulated concentrations of CRP, SAA, ferritin and IP-10 had better discriminating ability than markers from stimulated samples. Accuracy of marker combinations by general discriminant analysis (GDA) identified a six analyte model with 77% accuracy for TB cases and 84% for non TB cases, with a better performance in HIV uninfected patients. : A biosignature of 6 cytokines obtained after stimulation with four Mtb antigens has moderate potential as a diagnostic tool for pulmonary TB disease individuals and stimulated marker expression had no added value to unstimulated marker performance.
Databáze: OpenAIRE
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