| Autor: |
Ashton, David S., Beddell, Christopher R., Cooper, David J., Green, Brian N., Oliver, Ronald W.A., Welham, Kevin J. |
| Jazyk: |
angličtina |
| Předmět: |
|
| Zdroj: |
FEBS Letters. (1-2):201-204 |
| ISSN: |
0014-5793 |
| DOI: |
10.1016/0014-5793(91)80867-3 |
| Popis: |
Electrospray mass spectrometry has been used to measure the masses of the species present in solutions of three serine proteases (α-chymotrypsin, subtilisin Carlsberg and subtilisin BPN′) before, during and after completion of the hydrolytic reaction with cinnamoyl imidazole and indole acryloyl imidazole. The masses measured during the reaction demonstrated that covalentO-acyl enzyme intermediates had been formed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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