| Autor: |
Suárez, Nicolás M., Musonda, Kunda G., Escriva, Eric, Njenga, Margaret, Agbueze, Anthony, Camiolo, Salvatore, Davison, Andrew J., Gompels, Ursula A. |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| ISSN: |
0022-1899 |
| Popis: |
Background:\ud In developed countries, human cytomegalovirus (HCMV) is a major pathogen in congenitally infected and immunocompromised individuals, where multiple-strain infection appears linked to disease severity. The situation is less documented in developing countries. In Zambia, breast milk is a key route for transmitting HCMV and carries higher viral loads in human immunodeficiency virus (HIV)–infected women. We investigated HCMV strain diversity.\ud \ud Methods:\ud High-throughput sequence datasets were generated from 28 HCMV-positive breast milk samples donated by 22 mothers (15 HIV-infected and 7 HIV-negative) at 4–16 weeks postpartum, then analyzed by genome assembly and novel motif-based genotyping in 12 hypervariable HCMV genes.\ud \ud Results:\ud Among the 20 samples from 14 donors (13 HIV-infected and one HIV-negative) who yielded data meeting quality thresholds, 89 of the possible 109 genotypes were detected, and multiple-strain infections involving up to 5 strains per person were apparent in 9 HIV-infected women. Strain diversity was extensive among individuals but conserved compartmentally and longitudinally within them. Genotypic linkage was maintained within hypervariable UL73/UL74 and RL12/RL13/UL1 loci for virus entry and immunomodulation, but not between genes more distant from each other.\ud \ud Conclusions:\ud Breast milk from HIV-infected women contains multiple HCMV strains of high genotypic complexity and thus constitutes a major source for transmitting viral diversity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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