| Autor: |
Sanders, Michael R, Clifton, Luke A, Frazier, Richard A., Green, Rebecca J. |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| ISSN: |
0743-7463 |
| Popis: |
We have studied how puroindoline-b (PINB) mutants bind to model eukaryotic membranes dependent on binary composition of anionic:zwitterionic phospholipids and the presence of cholesterol and sphingomyelin in the model membrane. We have found that the trends in lipid binding behavior are different for wild-type PINB compared to its naturally occurring PINB(Trp44Arg) mutant form and have seen evidence of protein-induced domain formation within the lipid layer structure. Results show that selective binding of antimicrobial peptides to different membrane types is as a result of differences in lipid composition and the arrangement of lipids within the membrane surface. However, membrane-binding behavior is not easily predicted; it is determined by net charge, hydrophobicity, and the amphiphilicity of the protein/peptide lipid-binding domain. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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