| Autor: |
Hoffmann, Jedrzej, Shmeleva, Evgeniya, Boag, Stephen, Fiser, Karel, Bagnall, Alan, Murali, Santosh, Dimmick, Ian, Pircher, Hanspeter, Martin-Ruiz, Carmen, Egred, Mohaned, Keavney, Bernard, von Zglinicki, Thomas, Das, Rajiv, Todryk, Stephen, Spyridopoulos, Ioakim |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
|
| ISSN: |
0009-7330 |
| Popis: |
Rationale: There is mounting evidence of a higher incidence of coronary heart disease (CHD) in cytomegalovirus (CMV) seropositive individuals.\ud \ud Objective: The aim of this study was to investigate whether acute MI triggers an inflammatory T-cell response that might lead to accelerated immunosenescence in CMV-seropositive patients.\ud \ud Methods and Results: Thirty-four patients with acute MI undergoing primary PCI (PPCI) were longitudinally studied within 3 months following reperfusion (Cohort A). In addition, 54 patients with acute and chronic MI were analyzed in a cross-sectional study (Cohort B). CMV-seropositive patients demonstrated a greater fall in the concentration of terminally differentiated CD8 effector memory T cells (TEMRA) in peripheral blood during the first 30 min of reperfusion compared with CMV-seronegative patients (-192 vs. -63 cells/µl; p=0.008), correlating with the expression of programmed cell death-1 (PD-1) before PPCI (r=0.8; p=0.0002). A significant proportion of TEMRA cells remained depleted for at least 3 months in CMV-seropositive patients. Using high-throughput 13-parameter flow cytometry and HLA class I CMV-specific dextramers, we confirmed an acute and persistent depletion of terminally differentiated TEMRA and CMV-specific CD8+ cells in CMV-seropositive patients. Long-term reconstitution of the TEMRA pool in chronic CMV-seropositive post-MI patients was associated with signs of terminal differentiation including an increase in KLRG1 and shorter telomere length in CD8+ T cells (2225 bp vs. 3397 bp; p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
