| Autor: |
Dufour, Éric, Tam, Wendy, Nägler, Dorit K, Storer, Andrew C, Ménard, Robert |
| Jazyk: |
angličtina |
| Předmět: |
|
| Zdroj: |
FEBS Letters. (1-2):78-82 |
| ISSN: |
0014-5793 |
| DOI: |
10.1016/S0014-5793(98)00773-X |
| Popis: |
To demonstrate the usefulness of an engineered papain nitrile hydratase as a biocatalyst, a peptide amidrazone was prepared by incubation of the nitrile MeOCO-Phe-Ala-nitrile with the Gln19Glu papain mutant in the presence of salicylic hydrazide as a nucleophile. The amidrazone results from nucleophilic attack by salicylic hydrazide at the imino carbon of the thioimidate adduct formed between the enzyme and the peptide nitrile substrate. Compared to wild-type enzyme, the engineered nitrile hydratase causes a better than 4000-fold increase in the rate of amidrazone formation and yields a product of much higher purity. The advantages over other nitrile-hydrolyzing enzymes and current limitations of the papain nitrile hydratase are discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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