| Autor: |
Braks, J.A.M., Mair, G.R., Franke-Fayard, B., Janse, C.J., Waters, A.P. |
| Jazyk: |
angličtina |
| Rok vydání: |
2008 |
| Předmět: |
|
| ISSN: |
1362-4962 |
| Popis: |
Translational repression (TR) plays an important role\ud in post-transcriptional regulation of gene expression\ud and embryonic development in metazoans. TR\ud also regulates the expression of a subset of the\ud cytoplasmic mRNA population during development\ud of fertilized female gametes of the unicellular\ud malaria parasite, Plasmodium spp. which results\ud in the formation of a polar and motile form, the\ud ookinete. We report the conserved and sex-specific\ud regulatory role of either the 3’- or 5’-UTR of a subset\ud of translationally repressed mRNA species as\ud shown by almost complete inhibition of expression\ud of a GFP reporter protein in the female gametocyte.\ud A U-rich, TR-associated element, identified previously\ud in the 3’-UTR of TR-associated transcripts,\ud played an essential role in mediating TR and a\ud similar region could be found in the 5’-UTR shown in\ud this study to be active in TR. The silencing effect of\ud this 5’-UTR was shown to be independent of its\ud position relative to its ORF, as transposition to a\ud location 3’ of the ORF did not affect TR. These\ud results demonstrate for the first time in a unicellular\ud organism that the 5’ or the 3’-UTR of TR-associated\ud transcripts play an important and conserved role in\ud mediating TR in female gametocytes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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