| Autor: |
Peckham, R.K., Brill, R., Foster, David S., Bowen, A.L., Leigh, James A., Coffey, Tracey J., Flynn, Robin J. |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| ISSN: |
2045-2322 |
| Popis: |
IL-17 has emerged as a key player in the immune system, exhibiting roles in protection from infectious diseases and promoting inflammation in autoimmunity. Initially thought to be CD4 T-cell-derived, the sources of IL-17 are now known to be varied and belong to both the innate and adaptive arms of the immune system. Mechanisms for inducing IL-17 production in lymphoid cells are thought to rely on appropriate antigenic stimulation in the context of TGF-?1, IL-6 and/or IL-1?. Using culture protocols adapted from human studies, we have effectively induced both bovine CD4+ and WC1+ ?? T-cells to produce IL-17 termed Th17 and ??17 cells, respectively. The negative regulatory effect of IFN-? on mouse and human IL-17 production can be extended to the bovine model, as addition of IFN-? decreases IL-17 production in both cell types. Furthermore we show that infection with the protozoan Neospora caninum will induce fibroblasts to secrete pro-IL-17 factors thereby inducing a ??17 phenotype that preferentially kills infected target cells. Our study identifies two T-cell sources of IL-17, and is the first to demonstrate a protective effect of IL-17+ T-cells in ruminants. Our findings offer further opportunities for future adjuvants or vaccines which could benefit from inducing these responses. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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