UHPLC-MS/MS analysis of cocoa bean proteomes from four different genotypes

Autor: Scollo, Emanuele, Neville, David C. A., Oruna-Concha, M. Jose, Trotin, Martine, Cramer, Rainer
Jazyk: angličtina
Rok vydání: 2020
Předmět:
ISSN: 0308-8146
Popis: In this study the proteomic profiles of cocoa beans from four genotypes with different flavour profiles were analysed by bottom-up label-free UHPLC-MS/MS. From a total of 430 identified proteins, 61 proteins were found significantly differentially expressed among the four cocoa genotypes analysed with a fold change of ≥2. PCA analysis allowed clear separation of the genotypes based on their proteomic profiles. Genotype-specific abundances were recorded for proteases involved in the degradation of storage proteins and release of flavour precursors. Different genotype-specific levels of other enzymes, which generate volatiles compounds that could potentially lead to flavour-inducing compounds, were also detected. Overall, this study shows that UHPLC-MS/MS data can differentiate cocoa bean varieties.
Databáze: OpenAIRE