| Autor: |
Zafeiropoulou, Konstantina, Nichols, Ben, Mackinder, Mary, Biskou, Olga, Rizou, Eleni, Karanikolou, Antonia, Clark, Clare, Buchanan, Elaine, Cardigan, Tracey, Duncan, Hazel, Wands, David, Russell, Julie, Hansen, Richard, Russell, Richard K., McGrogan, Paraic, Edwards, Christine A., Ijaz, Umer Z., Gerasimidis, Konstantinos |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| ISSN: |
0016-5085 |
| Popis: |
Background & Aims: \ud It is not clear whether alterations in the intestinal microbiota of children with celiac disease cause the disease or are a result of disease and/or its treatment with gluten-free diet (GFD).\ud \ud Methods: \ud We obtained 167 fecal samples from 141 children (20 with new-onset celiac disease, 45 treated with a GFD, 57 healthy children, and 19 unaffected siblings of children with celiac disease) in Glasgow, Scotland. Samples were analyzed by 16S rRNA sequencing and diet-related metabolites were measured by gas chromatography. We obtained fecal samples from 13 of the children with new-onset CD after 6 and 12 months on GFD. Relationships between microbiota with diet composition, gastrointestinal function, and biomarkers of GFD compliance were explored.\ud \ud Results: \ud Microbiota α diversity did not differ among groups. Microbial dysbiosis was not observed in children with new-onset celiac disease. In contrast, 2.8% (Bray-Curtis dissimilarity index, P=.025) and 2.5% (UniFrac distances, P=.027) of the variation in microbiota composition could be accounted for by the GFD. Between 3% to 5% of all taxa differed among all group comparisons. Eleven distinctive operational taxonomic units composed a microbe signature specific to celiac disease with high diagnostic probability. Most of the operational taxonomic units that differed between patients on GFD with new-onset celiac disease vs healthy children were associated with nutrient and food group intake (from 75% to 94%), and with biomarkers of gluten ingestion. Fecal levels of butyrate and ammonia decreased during the GFD.\ud \ud Conclusions: \ud Although several alterations in the intestinal microbiota of children with established celiac disease appear to be effects of a GFD, there are specific bacteria that are distinct biomarkers of celiac disease. Studies are needed to determine whether these bacteria contribute to pathogenesis of celiac disease. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
