| Autor: |
Stoynov, Stoyno S, Bakalova, Anastassia T, Dimov, Svetoslav I, Mitkova, Atanaska V, Dolapchiev, Luben B |
| Jazyk: |
angličtina |
| Předmět: |
|
| Zdroj: |
FEBS Letters. (2):151-154 |
| ISSN: |
0014-5793 |
| DOI: |
10.1016/S0014-5793(97)00489-4 |
| Popis: |
Polyclonal antibodies against the exonuclease from Crotalus adamanteus venom (the 140-kDa protein) inhibit both the exonucleolytic and the single-strand-specific endonucleolytic activities, present in the exonuclease preparation. The antibodies also diminish the ability of the enzyme to split the negatively supercoiled Bluescript KS+ in the AT-rich fragment near-by the transcription termination site of the Ampicillin gene. Therefore the single-strand-specific endonucleolytic activity was attributed to the protein molecule of the exonuclease. The processivity of the exonucleolytic action was found to be less than 3 monomers as indicated by the heparin trapping method. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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