Controlled release of 18-beta-glycyrrhetic acid by nanodelivery systems increases cytotoxicity on oral carcinoma cell line
| Autor: | I. Cacciotti, L. Chronopoulou, C. Palocci, A. Amalfitano, M. Cantiani, M. Cordaro, C. Lajolo, C. Callà, A. Boninsegna, D. Lucchetti, P. Gallenzi, A. Sgambato, G. Nocca, A. Arcovito |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Zdroj: | Nanotechnology (Bristol, Online) (2018). info:cnr-pdr/source/autori:I. Cacciotti, L. Chronopoulou, C. Palocci, A. Amalfitano, M. Cantiani, M. Cordaro, C. Lajolo, C. Callà, A. Boninsegna, D. Lucchetti, P. Gallenzi, A. Sgambato, G. Nocca, A. Arcovito/titolo:Controlled release of 18-beta-glycyrrhetic acid by nanodelivery systems increases cytotoxicity on oral carcinoma cell line/doi:/rivista:Nanotechnology (Bristol, Online)/anno:2018/pagina_da:/pagina_a:/intervallo_pagine:/volume |
| Popis: | The topical treatment for oral mucosal diseases is often based on products optimized for dermatologic applications; consequently, a lower therapeutic effect may be present. 18-?-glycyrrhetic acid (GA) is extracted from Glycirrhiza glabra. The first aim of this study was to test the cytotoxicity of GA on PE/CA-PJ15 cells. The second aim was to propose and test two different delivery systems, i.e. nanoparticles and fibers, to guarantee a controlled release of GA in vitro. We used chitosan and poly(lactic-co-glycolic) acid based nanoparticles and polylactic acid fibers. We tested both delivery systems in vitro on PE/CA-PJ15 cells and on normal human gingival fibroblasts (HGFs). The morphology of GA-loaded nanoparticles (GA-NPs) and fibers (GA-FBs) was investigated by electron microscopy and dynamic light scattering; GA release kinetics was studied spectrophotometrically. MTT test was used to assess GA cytotoxicity on both cancer and normal cells. Cells were exposed to different concentrations of GA (20-500 ?mol l-1) administered as free GA (GA-f), and to GA-NPs or GA-FBs. ROS production was evaluated using dichlorodihydrofluorescein as a fluorescent probe. Regarding the cytotoxic effect of GA on PE/CA-PJ15 cells, the lowest TC50 value was 200 ?mol l-1 when GA was added as GA-NPs. No cytotoxic effects were observed when GA was administered to HGFs. N-acetyl Cysteine reduced mortality induced by GA-f in PE/CA-PJ15 cells. The specific effect of GA on PE/CA-PJ15 cells is mainly due to the different sensitivity of cancer cells to ROS over-production; GA-NPs and GA-FBs formulations increase, in vitro, this toxic effect on oral cancer cells |
| Databáze: | OpenAIRE |
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