PROTECTIVE EFFECT OF GROWTH FACTORS ON TISSUE TRASNGLUTAMINASE OVEREXPRESSION INDUCED BY ?-AMYLOID IN OLFACTORY ENSHEATHING CELLS. A STUDY IN VITRO
| Autor: | Roberta Bonfanti, Michela Spatuzza, Mariacristina Ferrara, Agata Campisi, Rosalia Pellitteri |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | XXV CONVEGNO NAZIONALE, Roma, 27-28 novembre 2015 info:cnr-pdr/source/autori:Roberta Bonfanti, Michela Spatuzza, Mariacristina Ferrara, Agata Campisi and Rosalia Pellitteri/congresso_nome:XXV CONVEGNO NAZIONALE/congresso_luogo:Roma/congresso_data:27-28 novembre 2015/anno:2015/pagina_da:/pagina_a:/intervallo_pagine |
| Popis: | Tissue transglutaminase (TG2) is a calcium dependent protein implicated in numerous physiological and pathological cellular processes, including signal transduction, cellular survival, differentiation, apoptosis, cancer and neurodegenerative diseases, such as Parkinson and Alzheimer diseases (AD). TG2 activity has been detected in normal and AD brains and it has been proposed that the protein is involved in development of abnormal insoluble neurofilaments. Furthermore, it has been demonstrated that Amyloid-beta (A?) is a substrate for TG2, which is a reactive acceptor and donor sites responsible for the TG-catalysed formation of polymers. In previous studies we demonstrated a relationship between TG2 and Growth Factors (GFs) in astrocytes and Olfactory Ensheathing Cells (OECs), a cell type capable of continous neurogenesis throughout lifetime. Specifically, we showed that TG2 overexpression induced by some stressors was down-regulated by GFs exposure in OECs. Herein, we assessed the effect of some GFs (bFGF and GDNF) on TG2 overespression induced by native full-length peptide (A? 1-42) or by fragments, such as A? (25-35) or A? (35-25, as control). To monitor cell viability, MTT test was used, whereas cell morphological features was examined by immunocytochemical procedures, using anti-Vimentin and anti-Caspase-3 cleavage. TG2 expression levels were evaluated immunocytochemically. Our results demonstrate that when OECs were exposed to A? (1-42) or A? (25-35) fragments for 24 h, TG2 expression was increased. The pre-treatment of cells with GFs significatively decreased the TG2 positive cells, when compared with the untreated controls. In addition, Vimentin expression in treated and untreated-OECs remained unchanged, while Caspase-3 cleavage was modulated by GFs in stressed cells with A? (1-42) or A? (25-35), when compared with the controls. Our data suggest that OECs exposed to GFs, which are able to modulate TG2 levels, could be an innovative mechanism to contrast TG2 expression, which plays a key role in AD. |
| Databáze: | OpenAIRE |
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