Functional characterisation of an ammonium transporter gene from Lotus japonicus

Autor: Salvemini F, Marini A, Riccio A, Patriarca EJ, Chiurazzi M.
Rok vydání: 2001
Předmět:
Zdroj: Gene (Amst.) 270 (2001): 237–243.
info:cnr-pdr/source/autori:Salvemini F, Marini A, Riccio A, Patriarca EJ, Chiurazzi M./titolo:Functional characterisation of an ammonium transporter gene from Lotus japonicus/doi:/rivista:Gene (Amst.)/anno:2001/pagina_da:237/pagina_a:243/intervallo_pagine:237–243/volume:270
Popis: NH4+ is the main product of symbiotic nitrogen fixation and the external concentration of combined nitrogen plays a key regulatory role in all the different step of plant-rhizobia interaction. We report the cloning and characterization of the first member of the ammonium transporter family, LjAMT1;1 from a leguminous plant, Lotus japonicus. Sequence analysis reveals a close relationship to plant transporters of the AMT1 family. The wild type and two mutated versions of LjAMT1;1 were expressed and functionally characterised in yeast. LjAMT1;1 is transcribed in roots, leaves and nodules of L. japonicus plants grown under low nitrogen conditions, consistent with a role in uptake of NH4+ by the plant cells.
Databáze: OpenAIRE