Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis and gene expression disregulation on HeLa cells
| Autor: | Pagliara P., Scarano A., Barca A., Verri T., Zuppone S., Caroppo C. |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Journal of applied phycology (Dordr., Online) 27 (2015): 2321–2332. doi:10.1007/s10811-014-0515-z. info:cnr-pdr/source/autori:Pagliara P., Scarano A., Barca A., Verri T., Zuppone S., Caroppo C./titolo:Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis and gene expression disregulation on HeLa cells/doi:10.1007%2Fs10811-014-0515-z./rivista:Journal of applied phycology (Dordr., Online)/anno:2015/pagina_da:2321/pagina_a:2332/intervallo_pagine:2321–2332/volume:27 |
| Popis: | The dinoflagellates of the genus Ostreopsis Schmidt are toxic species involved in the occurrence of massive blooms. These dinoflagellates are known to produce palytoxin and ovatoxins, which are considered amongst the most poisonous phycotoxins in the world. In this work, aqueous and methanolic extracts of one O. cf. ovata Ionian strain were tested on the human-derived HeLa cell line, and cytotoxicity, cytoskeletal rearrangment and apoptosis induction were recorded by morphological and molecular analysis. The MTT test revealed high toxicity for both extracts, which activity was inhibited by ouabain, which suggests the main involvement of palytoxin-like molecules in the observed toxic effects of this strain. Overall, the effects induced by O. cf. ovata on HeLa cells were dependent on the type of adopted extracts, being both toxic, but with the methanolic extract more toxic than the aqueous one and able to induce cell death by apoptosis. This process was evidenced by both phosphatidylserine exposition and up regulation of caspase-3 gene expression. The different effect of the two extracts on cell death is indicative of their different composition and/or activity. Our findings represent the starting step for the characterization of novel bioactive molecules. |
| Databáze: | OpenAIRE |
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