GENOTIPIZZAZIONE DI S. AUREUS ISOLATI DA LATTE BOVINO DI AZIENDE LOMBARDE
| Autor: | Gazzola A. [1], Romanò A. [1, Maisano A.M. [1], Spelta C. [1], Bertocchi L. [2], Ferrara G [2], Finazzi G. [3], Filipello V. [3], Decastelli L. [4], Bergagna S. [5], Cremonesi P. [6], Vezzoli F. [1], Graber HU. [7], Luini M.*[1] |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | XVIII Congresso Nazionale S.I.Di.L.V., Perugia, 7-9 Novembre 2018 info:cnr-pdr/source/autori:Gazzola A. [1], Romanò A. [1,7], Maisano A.M. [1], Spelta C. [1], Bertocchi L. [2], Ferrara G [2], Finazzi G. [3], Filipello V. [3], Decastelli L. [4], Bergagna S. [5], Cremonesi P. [6], Vezzoli F. [1], Graber HU. [7], Luini M.*[1]/congresso_nome:XVIII Congresso Nazionale S.I.Di.L.V./congresso_luogo:Perugia/congresso_data:7-9 Novembre 2018/anno:2018/pagina_da:/pagina_a:/intervallo_pagine |
| Popis: | Staphylococcus aureus strains (n=1559) isolated from North Italian dairy herds were genotyped by RS-PCR. A selection of these strains (84 from bulk tank milk and 91 from individual cow milk) was also analyzed for the presence of adlb gene, which is considered a possible marker of contagiousness. The adlb gene amplification was included in a multiplex PCR also targeting nuc and mecA genes. RS-PCR identified 83 different genotypes combined into 48 genotypic clusters, among which genotype B with his variants (GTB) was the most prevalent (n=491). A clonal diffusion of a single genotype was shown in most farms (373/458), especially when GTB was involved. Most of Staph. aureus belonging to GTB carried the adlb gene (38/43), as well as very few strains belonging to a different genotype. When investigated, all of them were associated with high prevalence in the herds, confirming that RS-PCR and adlb PCR could be useful tools in predicting the clinical properties of isolates. |
| Databáze: | OpenAIRE |
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