Circulating microRNA profiling in serum of pediatric patients with heart failure submitted to VAD implant
| Autor: | R. Ragusa (1), C. Caselli (2), M. G. Trivella (2), L. Pitto (2), M. Cabiati (2), S. Del Ry (2), A. Di Molfetta (3), A. Amodeo (3), M. Rizzo (4), R. D'Aurizio (5) |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | European heart journal 39 (2018). info:cnr-pdr/source/autori:R. Ragusa (1), C. Caselli (2), M. G. Trivella (2), L. Pitto (2), M. Cabiati (2), S. Del Ry (2), A. Di Molfetta (3), A. Amodeo (3), M. Rizzo (4), R. D'Aurizio (5)/titolo:Circulating microRNA profiling in serum of pediatric patients with heart failure submitted to VAD implant/doi:/rivista:European heart journal/anno:2018/pagina_da:/pagina_a:/intervallo_pagine:/volume:39 |
| Popis: | Methods: Blood samples were collected from 5 pediatric patients [13±6 (mean±SD) months, 17±2 LVEF%] with HF before and at 4 hrs, 1, 3, 7, 14 and 30 days after VAD implant. C-miRNA profile at VAD implant and after 30 days was determined by NGS. Modified c-miRNA were validated by qRT-PCR and assessed in blood samples collected during time-course after VAD implant. Putative miRNA targets were selected using miRWalk database. Results: After NGS, a total of 169 c-miRNAs were detected in serum samples of HF pediatric patients. N=13 c-miRNAs were simultaneously modulated at 30 days after VAD implant compared to pre-VAD (Table 1). Among them, only 6 cmiRNAs were confirmed by qRT-PCR (Fig. 1A). Putative targets of selected cmiRNAs seem to be involved with a synergistic effect in the hemostatic process(Fig. 1B). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|