EFFECTS OF WITHANIA SOMNIFERA EXTRACT ON PAIN BEHAVIOUR AND SPINAL CORD C-FOS EXPRESSION AFTER FORMALIN-INDUCED NOCICEPTION IN MICE
| Autor: | M.A. Casu, A. Orrù, G. Casu, S. Tambaro, G. Marchese, S. Ruiu |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Zdroj: | 23th National Congress of the "Gruppo Italiano Per Lo Studio Della Neuromorfologia" G.I.S.N., November 22-23, 2013, Cagliari, 22-23 novembre 2013 info:cnr-pdr/source/autori:M.A. Casu, A. Orrù, G. Casu, S. Tambaro, G. Marchese, S. Ruiu/congresso_nome:23th National Congress of the "Gruppo Italiano Per Lo Studio Della Neuromorfologia" G.I.S.N., November 22-23, 2013/congresso_luogo:Cagliari/congresso_data:22-23 novembre 2013/anno:2013/pagina_da:/pagina_a:/intervallo_pagine |
| Popis: | Withania somnifera Dunal (WS, family: Solanaceae) is a safe medicinal plant commonly used in Ayurvedic medicine to treat several diseases. Previous studies demonstrated that WS prevented the development of tolerance to the analgesic effect of morphine and suggested its potential anti-inflammatory properties. We recently found in mice that WS extract (WSE) prolonged morphine-induced anti-nociception and prevented the emergence of morphine-induced hyperalgesia although devoid of per se anti-nociceptive activity. To investigate further the potential role of WS in the modulation of pain, we evaluated the ability of WSE to modulate mice's anti-nociceptive responses in the formalin test. c-FOS protein expression in L4-L5 spinal dorsal horn was also analysed as a marker of neuronal activation following noxious stimulation. Male CD1 mice were pre-treated with 0, 100, 150 or 200 mg/Kg WSE (i.p.) 30 min before Formalin injection (20 µl of 5% diluted in saline) into the left hind paw. The time spent licking the formalin-injected paw was recorded for 45 min. Phases were defined as follows: Phase I (0-5 min), Phase II (10-45 min). Fos-immunoreactive (Fos-IR) spinal neurons were stained according to a standard avidin-biotin-peroxidase technique using a rabbit polyclonal antibody against c-Fos protein and a commercial ABC kit. Fos-IR neurons were visualized with 3,3diaminobenzidine. WSE was devoid of anti-nociceptive activity in the first acute phase of the formalin test, but interestingly, it reduced in a dosedependent manner the inflammatory response during the late phase. Consistently, WSE significantly decreased formalininduced Fos-IR in a dose-dependent manner in particular in the superficial layers. Together, the results confirm the potential anti-inflammatory activity of Withania somnifera extract. The mechanism of action WSE-induced anti-inflammatory activity is under investigation. |
| Databáze: | OpenAIRE |
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