Conformational properties, membrane interaction, and antibacterial activity of the peptaibiotic chalciporin A
| Autor: | Barbara Biondi Cristina Peggion Marta De Zotti Chiara Pignaffo Annalisa Dalzini Marco Bortolus Simona Oancea Geta Hilma Annalisa Bortolotti Lorenzo Stella Jens Z. Pedersen Victoria N. Syryamina Yuri D. Tsvetkov Sergei A. Dzuba Claudio Toniolo Fernando Formaggio |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Peptide Science Special Issue: Emerging Peptide Science in Italy (2018): e23083. doi:10.1002/bip.23083 info:cnr-pdr/source/autori:Barbara Biondi Cristina Peggion Marta De Zotti Chiara Pignaffo Annalisa Dalzini Marco Bortolus Simona Oancea Geta Hilma Annalisa Bortolotti Lorenzo Stella Jens Z. Pedersen Victoria N. Syryamina Yuri D. Tsvetkov Sergei A. Dzuba Claudio Toniolo Fernando Formaggio/titolo:Conformational properties, membrane interaction, and antibacterial activity of the peptaibiotic chalciporin A:..../doi:10.1002%2Fbip.23083/rivista:Peptide Science/anno:2018/pagina_da:e23083/pagina_a:/intervallo_pagine:e23083/volume:Special Issue: Emerging Peptide Science in Italy |
| DOI: | 10.1002/bip.23083 |
| Popis: | In this work, an extensive set of spectroscopic and biophysical techniques (including FT-IR absorption, CD, 2D-NMR, fluorescence, and CW/PELDOR EPR) was used to study the conformational preferences, membrane interaction, and bioactivity properties of the naturally occurring synthetic 14-mer peptaibiotic chalciporin A, characterized by a relatively low (?20%), uncommon proportion of the strongly helicogenic Aib residue. In addition to the unlabeled peptide, we gained in-depth information from the study of two labeled analogs, characterized by one or two residues of the helicogenic, nitroxyl radical-containing TOAC. All three compounds were prepared using the SPPS methodology, which was carefully modified in the course of the syntheses of TOAC-labeled analogs in view of the poorly reactive ?-amino function of this very bulky residue and the specific requirements of its free-radical side chain. Despite its potentially high flexibility, our results point to a predominant, partly amphiphilic, ?-helical conformation for this peptaibiotic. Therefore, not surprisingly, we found an effective membrane affinity and a remarkable penetration propensity. However, chalciporin A exhibits a selectivity in its antibacterial activity not in agreement with that typical of the other members of this peptide class. |
| Databáze: | OpenAIRE |
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